Journal:

Article Title: p16 INK4A as a marker for cervical dyskaryosis: CIN and cGIN in cervical biopsies and ThinPrep(TM) smears

doi:

Figure Lengend Snippet: Western blot analysis of p16INK4A protein extracted from CaSki cells using mouse monoclonal anti-p16INK4A antibody (clone G175–405). (A) Lane 1, molecular weight standards; lane 2, anti-p16INK4A clone G175–407. (B,C) Immunocytochemical analysis of cell line cytospins using anti-p16INK4A antibody (clone G175–405). Strong immunoreactivity was seen both in (B) CaSki and (C) C33A cervical carcinoma cell lines.

Article Snippet: Immunoreactive bands were detected with diaminobezaminidine (Vector Laboratories) (fig 1A ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Western blot analysis of p16 INK4A protein extracted from CaSki cells using mouse monoclonal anti-p16 INK4A antibody (clone G175–405). (A) Lane 1, molecular weight standards; lane 2, anti-p16 INK4A clone G175–407. (B,C) Immunocytochemical analysis of cell line cytospins using anti-p16 INK4A antibody (clone G175–405).

Techniques: Western Blot, Molecular Weight

Journal:

Article Title: p16 INK4A as a marker for cervical dyskaryosis: CIN and cGIN in cervical biopsies and ThinPrep(TM) smears

doi:

Figure Lengend Snippet: Comparison between p16 INK4A staining intensity and human papillomavirus (HPV) status

Article Snippet: Immunoreactive bands were detected with diaminobezaminidine (Vector Laboratories) (fig 1A ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Western blot analysis of p16 INK4A protein extracted from CaSki cells using mouse monoclonal anti-p16 INK4A antibody (clone G175–405). (A) Lane 1, molecular weight standards; lane 2, anti-p16 INK4A clone G175–407. (B,C) Immunocytochemical analysis of cell line cytospins using anti-p16 INK4A antibody (clone G175–405).

Techniques: Staining

Journal:

Article Title: p16 INK4A as a marker for cervical dyskaryosis: CIN and cGIN in cervical biopsies and ThinPrep(TM) smears

doi:

Figure Lengend Snippet: Immunohistochemical staining for p16INK4A in histologically normal tissue samples. (A) Normal epithelium, (B) squamous metaplasia, (C) normal endocervical gland, and (D) reactive epithelium showing chronic inflammation. Immunohistochemical staining for p16INK4A in cervical genital lesions. (E) CIN1, (F) CIN2, (G) CIN3 in an endocervical gland, and (H) cGIN.

Article Snippet: Immunoreactive bands were detected with diaminobezaminidine (Vector Laboratories) (fig 1A ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Western blot analysis of p16 INK4A protein extracted from CaSki cells using mouse monoclonal anti-p16 INK4A antibody (clone G175–405). (A) Lane 1, molecular weight standards; lane 2, anti-p16 INK4A clone G175–407. (B,C) Immunocytochemical analysis of cell line cytospins using anti-p16 INK4A antibody (clone G175–405).

Techniques: Immunohistochemical staining, Staining

Journal:

Article Title: p16 INK4A as a marker for cervical dyskaryosis: CIN and cGIN in cervical biopsies and ThinPrep(TM) smears

doi:

Figure Lengend Snippet: Scoring system for p16INK4A immunostaining in CIN3 lesions. (A) 0, (B) 1, (C) 2, and (D) 3. Immunohistochemical staining of p16INK4A in invasive squamous cell carcinoma; (E) nuclear staining, (F) cytoplasmic staining, (G) adenocarcinoma, and (H) cGIN. Immunocytochemical staining of exfoliated dysplastic cells in ThinPrep smears using p16INK4A specific antibody; (I) p16INK4a expression in a mild dyskaryotic cell; (J) mild to moderately dyskaryotic cell cluster exhibiting p16INK4a immunopositivity.

Article Snippet: Immunoreactive bands were detected with diaminobezaminidine (Vector Laboratories) (fig 1A ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Western blot analysis of p16 INK4A protein extracted from CaSki cells using mouse monoclonal anti-p16 INK4A antibody (clone G175–405). (A) Lane 1, molecular weight standards; lane 2, anti-p16 INK4A clone G175–407. (B,C) Immunocytochemical analysis of cell line cytospins using anti-p16 INK4A antibody (clone G175–405).

Techniques: Immunostaining, Immunohistochemical staining, Staining, Expressing